CONSISTENCY  MODERNIZED

CFX Opus Real-Time PCR Systems are the next evolution in quantitative PCR  (qPCR) from Bio-Rad Laboratories. With improved thermal performance and our  proprietary, accurate optical shuttle system, your data will be more consistent.  A sleek, modern design includes a refreshed and easy-to-use interface with more  flexible connectivity options for data management and instrument control. 

 

HAVE CONFIDENCE IN YOUR ENTIRE GENOMICS WORKFLOW

Unlike many suppliers, Bio-Rad offers everything you need for real-time PCR experiments, from sample
preparation to data analysis. Simplify and optimize your workflow with our robust suite of reagents,
instruments, and software, so that you can get publication-quality data every time.

 

Cell Lysis and RNA Isolation

■ Aurum Total RNA Mini Kit
■ Aurum Total RNA 96 Kit
■ Aurum Total RNA Fatty and Fibrous Tissue Kit
■ PureZOL RNA Isolation Reagent
■ SingleShot Cell Lysis Kits

 

Target Selection

■ PrimePCR Assays
■ PrimePCR Pathway Panels
■ PrimePCR Custom Plates

 

Reaction Setup

■ SsoAdvanced Universal Supermixes
■ iTaq Universal Supermixes
■ Reliance One-Step Multiplex Supermix
■ iTaq One-Step Universal Kits
■ SingleShot Cell Lysis RT-qPCR Kits
■ iScript Reverse Transcription Supermix for RT-qPCR
■ iScript gDNA Clear cDNA Synthesis Kit
■ PCR Plastics

 

Detection

■ CFX Family of Real-Time PCR Systems

 

Analysis

■ CFX Maestro Desktop Software
■ BR.io Web-Based Cloud Platform

 

More Connectivity Options than Ever

Bio-Rad recognizes that you need instruments that are as
connected as you are. In addition to Ethernet connectivity,
CFX Opus Systems are able to connect directly to a wireless
network (WiFi) for completely wireless operation and data retrieval.
Need flexibility? CFX Opus Systems are capable of stand-alone
operation and direct data transfer to a USB memory device or can
be connected to a PC.

With internet connectivity, CFX Opus Systems can be
programmed to send your data and any notifications directly
to your email, eliminating the need to check on the machine
during the run. New network drive access also allows you to
save your data directly to your local network* for easy access
by collaborators or from other network-connected locations.

CFX Opus Systems can also be linked to Bio-Rad’s
BR.io cloud platform, providing a modern, connected workflow
for the instrument.

 

Flexibility: Setup and Analysis Where and When You Want
Work Anywhere with the BR.io Cloud Platform

BR.io, Bio-Rad’s new cloud data management and analysis platform,
seamlessly integrates with CFX Opus Systems to provide remote
setup, instrument monitoring, and data management capabilities
without the need for an instrument-connected PC. The BR.io cloud
platform can be accessed from any computer using a Safari or
Chrome web browser, with no software installation required. Get the
most out of your instrument by setting up experiments and retrieving data remotely, minimizing time needed at the instrument. Your data are stored securely in the cloud, where they can be viewed and analyzed anywhere you have internet access.

 

CFX Maestro Software for Powerful Data Analysis
For more advanced data analysis, turn to CFX Maestro Software for
CFX Real-Time PCR Systems. CFX Maestro Software is designed
to work seamlessly with CFX Opus Systems for creation of
publication-quality figures and automatic statistical analysis.

 

POWERFUL SOFTWARE

With CFX Maestro Software You Can:

■ Perform automatic statistical analysis in seconds — runt-tests or analyze your data using one-way analysis of variance(ANOVA) with just a few mouse clicks

■ Extract more meaningful information from each run —analyze data using bar charts, box and whisker plots, dot plots,clustergrams, scatter plots, or volcano plots

■ Create and export publication-ready graphics — annotategraphs with P values, text, and arrows to call out specific data.Change colors, fonts, and legends. Export graphs at any sizeand resolution for presentations, posters, or publication

■ Integrate PrimePCR Assays easily — save time withpredesigned and validated PrimePCR Primers and Plates. Dragand drop your PrimePCR worksheet into CFX Maestro Softwareto instantly create your plate setup. Post-run, check run qualitywith the PrimePCR controls analysis tool

■ Work anywhere, on a PC or Mac — with both PC and Macversions of CFX Maestro, you can analyze your data on your owncomputer, anytime, without the need for an internet connection(Mac version is for data analysis only and does not provideinstrument control)

■ Perform further data analysis using qbase+ Software —CFX Maestro Software comes with a premium license for qbase+Software to further enhance your data analysis capabilities

 

Precision Melt Analysis Software

Precision Melt Analysis Software imports and analyzes data files
generated by the CFX family of Real-Time PCR Systems to genotype
DNA samples based on their thermal denaturation. The software can
be used for a variety of applications, including gene variant discovery, single nucleotide polymorphism (SNP) screening, identification of insertions, deletions, or other unknown mutations, and analysis of methylated DNA percentage in unknown samples.

 

The Security You Need

The Security Edition of CFX Maestro Software integrates the
power of CFX Opus Real-Time PCR Systems with tools that allow
U.S. FDA 21 CFR Part 11 compliance.

 

Have confidence in the security of your data, thanks to:
■ Mandatory password-protected login — valid Windows
User Profile (local or directory) and password are required
■ Hardware protection key (HASP HL key) — key must be
attached to a USB port on the computer to use the software
■ File encryption — files cannot be opened or edited using
other programs
■ Automatic file checking — integrity and validity are checked
each time a file is opened
■ Electronic signatures — more than one electronic signature
can be applied to any file that can be opened with the software
■ Time- and date-stamped audit trails — read-only information
in the audit trail can be viewed only while the data file is open

 

UNIFORM THERMAL CYCLING

Superior Uniformity

We took a great block and made it even better.The CFX Opus Systems use an improved version ofthe CFX Touch Systems’ block to offer exceptionalperformance and uniformity while keeping compatibilitywith our consumables. With the best thermal uniformityand accuracy Bio-Rad has ever produced, you’ll neverworry about using the block from edge to edge again,even with highly sensitive assays.

Efficient Optimization

Determining the optimal temperature for primer annealingis crucial for efficient and specific target amplification.The thermal gradient feature of the CFX Opus Systemsallows you to optimize your assay in a single experiment,minimizing the use of precious samples and reagents andsaving valuable research time. At any step in a protocol,users can program a temperature gradient of up to 24°Cacross the reaction block, with exceptional temperatureuniformity and reproducibility within each gradient zone.

 

ROBUST OPTICAL DESIGN

The solid-state optical technology of the CFX Opus Systemsprovide sensitive detection for precise quantification and targetdiscrimination. Scanning just above the sample plate, the opticsshuttle individually illuminates and detects fluorescence from eachwell with high sensitivity and consistency. In either acquisitionmode, the optical system automatically collects data from all wells,so you can enter or edit well information on your own schedulewithout fear of losing data to annotation mistakes.

 

Five-Target Multiplexing*

The CFX Opus 96 System can discriminate between up to fivetargets in a single reaction well. The optical filter sets are designedto maximize fluorescence detection for specific dyes in specificchannels. At every position and with every scan, the optics shuttleis reproducibly centered above each well so the light path is alwaysfixed and optimal. There is no need to sacrifice data collection inone of the channels to normalize to a passive reference. TheCFX Opus 384 System offers all of these benefits with up to fourtargets per well.

 

Multiple Data Acquisition Modes

The CFX Opus Systems can acquire data using several modes.Choose to acquire data for SYBR® Green I, EvaGreen®, andsingle-color FAM protocols using the fast scan mode or chooseto acquire data from all channels when performing multiplexprotocols. The CFX Opus Systems include one channel with anLED-filter photodiode combination designated for single-colorfluorescence resonance energy transfer (FRET) experiments,further expanding your experimental options. FRET mode canexpand your experimental options to applications such as proteinthermal shift (melt) analysis.

 

Ordering Information

Catalog #	Description
12011319	CFX Opus 96 Real-Time PCR System, includes CFX Opus 96 System and communication cables. Order CFX Maestro Software separately
17005940	CFX Opus 96 Real-Time PCR System with Starter Package, includes CFX Opus 96 System, CFX Maestro Software, license for qbase+ Software, communication cables, reagents, consumables
12011452	CFX Opus 384 Real-Time PCR System, includes CFX Opus 384 System and communication cables. Order CFX Maestro Software separately
17005939	CFX Opus 384 Real-Time PCR System with Starter Package, includes CFX Opus 384 System, CFX Maestro Software, license for qbase+ Software, communication cables, reagents, consumables
12013758	CFX Maestro Software Version 2.0, compatible with Windows 7 and 10, includes USB installation drive, video quick guides, PDF instruction manual, license for qbase+ Software
12004128	CFX Maestro Software Version 1.1, Mac Edition, compatible with Mac OS X El Capitan and macOS Sierra, includes USB installation drive, video quick guides, PDF instruction manual, license for qbase+ Software; does not support instrument control
12012832	CFX Maestro Software Version 2.0, Security Edition, includes 1 user license, USB installation drive, HASP HL key
12013028	CFX Maestro Software Version 2.0, Security Edition, includes 5 user licenses, 5 USB installation drives, 5 HASP HL keys

 

Bio-Rad CFX OPUS RT-PCR Detection System